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Detection of the fungus Bretziella fagacearum in wood chip samples from healthy and infected oak trees. The wood chip samples were treated to extract and purify the fungal DNA which was then amplified using LAMP. The product of the LAMP assay was characterized using fluorospectroscopy. The results show high specificity and sensitivity. (a) Results from LAMP assay conducted using StepOnePlus™ Real-Time PCR System for heating and fluorescence detection. (b) Results from LAMP assay conducted using Thermo Scientific™ <t>Compact</t> <t>Digital</t> <t>Dry</t> <t>Bath/Block</t> <t>Heater</t> for heating and Agilent-Cary Eclipse Fluorescence Spectrophotometer for fluorescence detector. The difference in the fluorescence intensity ranges in each set of experiments reflects the varied sensitivities of the fluorescence devices used
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Detection of the fungus Bretziella fagacearum in wood chip samples from healthy and infected oak trees. The wood chip samples were treated to extract and purify the fungal DNA which was then amplified using LAMP. The product of the LAMP assay was characterized using fluorospectroscopy. The results show high specificity and sensitivity. (a) Results from LAMP assay conducted using StepOnePlus™ Real-Time PCR System for heating and fluorescence detection. (b) Results from LAMP assay conducted using Thermo Scientific™ <t>Compact</t> <t>Digital</t> <t>Dry</t> <t>Bath/Block</t> <t>Heater</t> for heating and Agilent-Cary Eclipse Fluorescence Spectrophotometer for fluorescence detector. The difference in the fluorescence intensity ranges in each set of experiments reflects the varied sensitivities of the fluorescence devices used
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Detection of the fungus Bretziella fagacearum in wood chip samples from healthy and infected oak trees. The wood chip samples were treated to extract and purify the fungal DNA which was then amplified using LAMP. The product of the LAMP assay was characterized using fluorospectroscopy. The results show high specificity and sensitivity. (a) Results from LAMP assay conducted using StepOnePlus™ Real-Time PCR System for heating and fluorescence detection. (b) Results from LAMP assay conducted using Thermo Scientific™ Compact Digital Dry Bath/Block Heater for heating and Agilent-Cary Eclipse Fluorescence Spectrophotometer for fluorescence detector. The difference in the fluorescence intensity ranges in each set of experiments reflects the varied sensitivities of the fluorescence devices used

Journal: Plant Methods

Article Title: A rapid LAMP assay for the diagnosis of oak wilt with the naked eye

doi: 10.1186/s13007-024-01254-8

Figure Lengend Snippet: Detection of the fungus Bretziella fagacearum in wood chip samples from healthy and infected oak trees. The wood chip samples were treated to extract and purify the fungal DNA which was then amplified using LAMP. The product of the LAMP assay was characterized using fluorospectroscopy. The results show high specificity and sensitivity. (a) Results from LAMP assay conducted using StepOnePlus™ Real-Time PCR System for heating and fluorescence detection. (b) Results from LAMP assay conducted using Thermo Scientific™ Compact Digital Dry Bath/Block Heater for heating and Agilent-Cary Eclipse Fluorescence Spectrophotometer for fluorescence detector. The difference in the fluorescence intensity ranges in each set of experiments reflects the varied sensitivities of the fluorescence devices used

Article Snippet: To show that LAMP can be done without a sophisticated PCR thermocycler, a set of experiments were also conducted using Thermo Scientific™ Compact Digital Dry Bath/Block Heater for incubation and Agilent-Cary Eclipse Fluorescence Spectrophotometer for fluorescence detection.

Techniques: Infection, Amplification, Lamp Assay, Real-time Polymerase Chain Reaction, Fluorescence, Blocking Assay, Spectrophotometry